Penentuan Metode Terbaik Uji Fosfor Untuk Tanaman Tomat Pada Tanah Inceptisols

. Izhar, L, Susila, AD, Purwoko, BS, Sutandi, A, and Mangku, IW. 2012. Determination of the Best Method of Soil P Test for Tomato (Lycopersicon esculentum Mill. L) on Inceptisols Soil. Phosphorus is one of important soil elements and application of the element in suitable concentration give high effect on tomato growth. A study on phosphorus analysis and its application for recommendation of soil fertilization of tomato cultivation on Inceptisols soil was conducted at the field and Greenhouse of Cikabayan, Bogor Agricultural University, from March to November 2010. The objective of this research was to obtain the best extraction method of soil-P test for determining phosphorus nutrient required for tomato cultivation on Inceptisol soil. Rate of phosphorus of 0X, ¼ X, ½ X, ¾ X, and 1X, where X was 368.5 kg/ha P2O5 with four replications, was applied in the study. The treatments were applied 6 months before planting date. The research was arranged in randomized complete block design. Analysis of correlation between soil-P and plant growth based on data collected from the plants grown in the greenhouse using incubation media in treated-soil was analyzed. Soil-P test was carried out by using five extraction methods i.e. Bray I (HCl 0,025 N + NH4F 0.03), Bray II (NH4F 0.03 N + HCl 0.10 N), Mehlich I (HCl 0.05 N + H2SO4 0.025 N), Morgan Wolf (NaC2H2H3O2.3H2O; pH 4.8), and Truogh [HCl 0.10 N + (NH4)2SO4; pH 3]. The results showed that there were significant differences among the treatments of P fertilizer on the variables of plant height, leaf number, and stem diameter of tomato. Biomass fresh and dry weight of tomato also showed significantly different between the treatments applied. The highest correlation was shown on Mehlich I extraction reagent between plant dry and fresh weight. It means that, this P-nutrient extraction method was the most appropriate in determining phosphorus nutrient for tomatoes on Inceptisols soil with a coefficient correlation of 0.88. Mehlich I can also be used to develop a comprehensive phosphorus fertilizer recommendation for tomato cultivation on Inceptisols soil (r value = 0.89)

Review of EEG and ERP studies of extraversion personality for baseline and cognitive tasks

According to psychological studies, the most fundamental personality is the extraversion personality. Most studies looking at differences between extroverts and introverts are pen and paper based studies. However, in a few studies, electrophysiological signals were involved. In this paper, we reviewed studies examining extraversion personality using electroencephalography (EEG) and event-related potentials (ERP). It was found that some of the EEG studies claimed that extroverts and introverts can be differentiated using baseline EEG, while some others claimed otherwise. Conflicting findings were also observed in the ERP studies; higher/lower P300 amplitude in extroverts compared to that of introverts in visual stimuli tasks. These various findings are probably due to differences in their experimental protocols, sample size, or age of subjects. Other possible reasons include no consideration given on the main feature of extraversion and the studies only focused on EEG power spectral analysis. We are thus suggesting for future investigations to involve the main feature such as sociability and/or to incorporate more EEG features in the analysis to produce more robust and reliable results. This review constitutes a guidance for research on brain-related conditions of extroverts and introverts and shall be useful in many areas

Penentuan Metode Terbaik Uji Kalium untuk Tanaman Tomat pada Tanah Inceptisols

Penelitian tentang studi analisis kalium tanah dan aplikasi pupuk kalium pada budidaya tomat pada tanah Inceptisols dilakukan di Kebun Percobaan dan Rumah Kaca di Cikabayan, Institut Pertanian Bogor, dari Bulan April hingga November 2011. Tujuan penelitian untuk mendapatkan metode ekstraksi kalium tanah yang terbaik guna menentukan dosis pupuk kalium pada budidaya tomat pada tanah Inceptisols. Penelitian menggunakan rancangan acak kelompok dengan perlakuan pemberian pupuk kalium pada beberapa tingkat dosis, yaitu 0, ¼, ½, ¾, dan 1X, dimana nilai X ialah 608,6 kg K O kg/ha dengan empat ulangan. Perlakuan pemupukan kalium diterapkan pada 3 bulan sebelum penanaman tomat. Analisis korelasi dilakukan antara kandungan K tanah dan pertumbuhan tanaman di dalam rumah kaca menggunakan media inkubasi berasal dari tanah setelah diberi perlakuan. Uji kalium tanah menggunakan lima metode ekstraksi, yaitu metode HCl 25%, NH422) OAc 1 M pH 7, Mehlich I, Truog, dan Morgan Vanema. Hasil penelitian menunjukkan perbedaan yang nyata antara pengaruh perlakuan pupuk K terhadap parameter tinggi tanaman, jumlah daun, dan diameter batang tomat. Bobot segar biomassa dan bobot kering tomat juga menunjukkan perbedaan pengaruh yang nyata antarperlakuan. Nilai korelasi tertinggi ditunjukkan pada metode pengekstrak Truog melalui parameter bobot kering dan basah relatif tanaman (r = 0,7). Dengan demikian, uji K tanah menggunakan metode Truog dapat digunakan sebagai metode ekstraksi yang paling tepat untuk menganalisis unsur hara kalium dalam rangka penyediaan rekomendasi pemupukan K pada budidaya tomat pada tanah Inceptisols

Characterization of callase (β-1,3-d-glucanase) activity during microsporogenesis in the sterile anthers of Allium sativum L. and the fertile anthers of A. atropurpureum

We examined callase activity in anthers of sterile Allium sativum (garlic) and fertile Allium atropurpureum. In A. sativum, a species that produces sterile pollen and propagates only vegetatively, callase was extracted from the thick walls of A. sativum microspore tetrads exhibited maximum activity at pH 4.8, and the corresponding in vivo values ranged from 4.5 to 5.0. Once microspores were released, in vitro callase activity peaked at three distinct pH values, reflecting the presence of three callase isoforms. One isoform, which was previously identified in the tetrad stage, displayed maximum activity at pH 4.8, and the remaining two isoforms, which were novel, were most active at pH 6.0 and 7.3. The corresponding in vivo values ranged from pH 4.75 to 6.0. In contrast, in A. atropurpureum, a sexually propagating species, three callase isoforms, active at pH 4.8–5.2, 6.1, and 7.3, were identified in samples of microsporangia that had released their microspores. The corresponding in vivo value for this plant was 5.9. The callose wall persists around A. sativum meiotic cells, whereas only one callase isoform, with an optimum activity of pH 4.8, is active in the acidic environment of the microsporangium. However, this isoform is degraded when the pH rises to 6.0 and two other callase isoforms, maximally active at pH 6.0 and 7.3, appear. Thus, factors that alter the pH of the microsporangium may indirectly affect the male gametophyte development by modulating the activity of callase and thereby regulating the degradation of the callose wall

Carfilzomib and dexamethasone versus bortezomib and dexamethasone for patients with relapsed or refractory multiple myeloma (ENDEAVOR): And randomised, phase 3, open-label, multicentre study

Background: Bortezomib with dexamethasone is a standard treatment option for relapsed or refractory multiple myeloma. Carfilzomib with dexamethasone has shown promising activity in patients in this disease setting. The aim of this study was to compare the combination of carfilzomib and dexamethasone with bortezomib and dexamethasone in patients with relapsed or refractory multiple myeloma. Methods: In this randomised, phase 3, open-label, multicentre study, patients with relapsed or refractory multiple myeloma who had one to three previous treatments were randomly assigned (1:1) using a blocked randomisation scheme (block size of four) to receive carfilzomib with dexamethasone (carfilzomib group) or bortezomib with dexamethasone (bortezomib group). Randomisation was stratified by previous proteasome inhibitor therapy, previous lines of treatment, International Staging System stage, and planned route of bortezomib administration if randomly assigned to bortezomib with dexamethasone. Patients received treatment until progression with carfilzomib (20 mg/m2 on days 1 and 2 of cycle 1; 56 mg/m2 thereafter; 30 min intravenous infusion) and dexamethasone (20 mg oral or intravenous infusion) or bortezomib (1·3 mg/m2; intravenous bolus or subcutaneous injection) and dexamethasone (20 mg oral or intravenous infusion). The primary endpoint was progression-free survival in the intention-to-treat population. All participants who received at least one dose of study drug were included in the safety analyses. The study is ongoing but not enrolling participants; results for the interim analysis of the primary endpoint are presented. The trial is registered at ClinicalTrials.gov, number NCT01568866. Findings: Between June 20, 2012, and June 30, 2014, 929 patients were randomly assigned (464 to the carfilzomib group; 465 to the bortezomib group). Median follow-up was 11·9 months (IQR 9·3-16·1) in the carfilzomib group and 11·1 months (8·2-14·3) in the bortezomib group. Median progression-free survival was 18·7 months (95% CI 15·6-not estimable) in the carfilzomib group versus 9·4 months (8·4-10·4) in the bortezomib group at a preplanned interim analysis (hazard ratio [HR] 0·53 [95% CI 0·44-0·65]; p<0·0001). On-study death due to adverse events occurred in 18 (4%) of 464 patients in the carfilzomib group and in 16 (3%) of 465 patients in the bortezomib group. Serious adverse events were reported in 224 (48%) of 463 patients in the carfilzomib group and in 162 (36%) of 456 patients in the bortezomib group. The most frequent grade 3 or higher adverse events were anaemia (67 [14%] of 463 patients in the carfilzomib group vs 45 [10%] of 456 patients in the bortezomib group), hypertension (41 [9%] vs 12 [3%]), thrombocytopenia (39 [8%] vs 43 [9%]), and pneumonia (32 [7%] vs 36 [8%]). Interpretation: For patients with relapsed or refractory multiple myeloma, carfilzomib with dexamethasone could be considered in cases in which bortezomib with dexamethasone is a potential treatment option. Funding: Onyx Pharmaceuticals, Inc., an Amgen subsidiary

PrimPol-dependent single-stranded gap formation mediates homologous recombination at bulky DNA adducts

Stalled replication forks can be restarted and repaired by RAD51-mediated homologous recombination (HR), but HR can also perform post-replicative repair after bypass of the obstacle. Bulky DNA adducts are important replication-blocking lesions, but it is unknown whether they activate HR at stalled forks or behind ongoing forks. Using mainly BPDE-DNA adducts as model lesions, we show that HR induced by bulky adducts in mammalian cells predominantly occurs at post-replicative gaps formed by the DNA/RNA primase PrimPol. RAD51 recruitment under these conditions does not result from fork stalling, but rather occurs at gaps formed by PrimPol re-priming and resection by MRE11 and EXO1. In contrast, RAD51 loading at double-strand breaks does not require PrimPol. At bulky adducts, PrimPol promotes sister chromatid exchange and genetic recombination. Our data support that HR at bulky adducts in mammalian cells involves post-replicative gap repair and define a role for PrimPol in HR-mediated DNA damage tolerance

Role of Follicle-Stimulating Hormone and Androgens on the Sexual Inversion of Sevenband Grouper Epinephelus septemfasciatus

This study was designed to characterize the amount of androgen implant and the time period necessary to cause sexual inversion in sevenband grouper Epinephelus septemfasciatus and to determine whether administration of follicle-stimulating hormone (FSH) with or without androgens had any effect on the sexual state of this species. Seventy-two fish were treated with 10 μg of recombinant FSH/kg body weight (BW) or saline control in cocoa butter. Two days later, cocoa butter and α-cellulose pellet implants of control, 0.1 mg of low-androgen mixture (LoAN)/kg BW, or 5 mg of high-androgen mixture (HiAN)/kg BW were injected into the same fish. Fish were sampled after 3 and 5 weeks. Gonadosomatic indices (GSIs) at 3 weeks for FSH, HiAN, and FSH+HiAN treatment groups were significantly lower than the control values. At 5 weeks, no significant difference was observed, although the GSIs for HiAN treatment groups were lowest at both sampling points. The highest GSI values recorded were in the FSH-only treatment group at 5 weeks. All the controls remained in the immature female state throughout the study. High-dose androgen levels caused sexual transition of gonadal tissue at 3 weeks with spermatocytes present in all individuals. At 5 weeks, all individuals in the HiAN treatment group had complete inversion to male gonad, and sperm sinus development occurred in four individuals. However, incomplete sexual inversion was observed in the FSH+HiAN treatment groups with some ovarian tissue persisting even at 5 weeks. Exogenous FSH treatment alone appears to stimulate ovarian growth in the postspawning period. The 3-week period of sexual transition in this study is one of the shortest time periods recorded for grouper sexual inversion. This may be due to androgen treatment in the postspawning period, the proposed period of natural sex change in several grouper species